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hybridization buffer  (Molecular Instruments)

 
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    Molecular Instruments hybridization buffer
    Hybridization Buffer, supplied by Molecular Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hybridization+buffer/buffer+hybridization+probe/us12655444-546-78-70
    Average 86 stars, based on 1 article reviews
    hybridization buffer - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Hybridization:

    Article Title: Evolutionary origins and transcriptomic innovations of vertebrate Cajal-Retzius cells.
    Article Snippet: .. After rinsing in SSCT, sections were pre-hybridized in Hybridization Buffer (Molecular Instruments) for 1.5h at 37◦C and then incubated overnight with probes at 12 nM concentration in Hybridization Buffer at 37◦C. .. The following day, samples were washed in Wash Buffer (Molecular Instruments) and then in SSCT, then incubated in Amplification Buffer (Molecular Instruments) for 30 min.

    Article Title: Artificial expression constructs for selectively modulating gene expression in non-neuronal brain cells
    Article Snippet: For sectioning, hemispheres were embedded in 1% low-melt agarose in PBS and cut 50 μm sagittal sections on a Leica VT1000S vibratome in cold PBS buffer. .. Sections were post-fixed in 4% PFA in PBS for 2 hours and then rinsed in PBS at room temperature, then dehydrated with 70% ethanol at 4° C. Afterwards sections could be stored for up to a month in 4° C. For staining, sections were cleared with 8% SDS in PBS for 2 hours at room temperature then washed three times in 2×SSC for 1 hour each, then with Hybridization Buffer (Molecular Instruments) in a new well before applying Hybridization Buffer containing HCR Probes and hybridized overnight at 37° C. The next day samples were washed with 30% Probe Wash Buffer for 1 hour at 37° C., then rinsed with 2×SSC. ..

    Article Title: Retinoic Acid Informs the Positional Identity of Frontonasal Neural Crest Cells Through Alx Family of Transcription Factors
    Article Snippet: .. After pre- hybridizing in 500 μL hybridization buffer (HCR Buffers, Molecular Instruments, USA) for 30 min at 37°C, the embryos were incubated overnight at 37°C in hybridization solution containing probes targeting genes (4 pmol in 500 μL hybridization buffer). .. After hybridization, samples were washed four times using HCR washing buffer (HCR Buffers, Molecular Instruments, USA) at 37°C, followed by two washes in 5xSSCT at RT.

    Article Title: Engulfment by brain macrophages in a short-lived vertebrate
    Article Snippet: .. After the overnight incubation with probes sets diluted in hybridization buffer, HCR wash buffer (Molecular Instruments, “HCR Probe Wash Buffer,” for tissue sections; 250 μL per slide; preheated to 37°C) was applied to was applied to the slides, which were incubated at 37°C for two washes of 30 minutes each with a coverslip on top. ..

    Article Title: Elevated phagocytic capacity directs innate spinal cord repair.
    Article Snippet: .. For blocking, sections were incubated in prewarmed hybridization buffer (Molecular Instruments) for 1 h at 37◦C. ..

    Article Title: Methods, culture medias and devices for generating embryos in vitro from stem cells
    Article Snippet: After at least one day of storage at −20° C., the tissue sections were permeabilized in 70% ethanol at 4° C. for >1 h, and then dried and cleared with 1 ml of 8% SDS (Invitrogen, AM9822) in 1×PBS at room temperature for 30 min. After rinsing with PBS two times and nuclease-free water one time, a custom-made flow cell (fluidic volume about 30 μl), which were made from glass slide (25×75 mm) with 1 mm thickness and 1 mm diameter holes and a PET film coated on both sides with an acrylic adhesive with total thickness of 0.25 mm (Grace Bio-Labs, RD481902) was attached to the coverslips. .. Using the 1 mm diameter holes, the tissue samples were incubated in 30% hybridization buffer (Molecular Instruments) containing 3.3 nM of each probe overnight at 37° C. The holes of the flow cells were covered using a sticker (Grace Bio-Labs, GBL629200) to prevent evaporation during incubation. .. The samples were then washed with 30% wash buffer (Molecular Instruments) for 4 times over 1 h, and then rinsed with 4× saline-sodium citrate buffer (SSC) (Thermo Fisher, 15557036) 5 times.

    Article Title: Evolutionary origins and transcriptomic innovations of vertebrate Cajal-Retzius cells.
    Article Snippet: .. Probes were added to the Hybridization Buffer (Molecular Instruments) to a concentration of 12 nM and incubated for three days at 37◦C. .. Samples were washed in Wash Buffer (Molecular Instruments) and SSCT (5xSSC + 0.1% TritonX) before incubation with 60 pM snap-cooled HCR hairpins (Molecular Instruments) in Amplification Buffer (Molecular Instruments) for 3 days at 4◦C.

    Article Title: An antagonistically pleiotropic gene regulates vertebrate growth, maturity, and lifespan.
    Article Snippet: In brief, after rehydration, slides were boiled for 15min in 0.01M citrate buffer (Sigma-Aldrich, C8532) and permeabilized with 20 μg/ml proteinase K (A&A Biotechnology, 1019-20-5) in PBS for 15min at 37 °C, before being washed with PBS. .. Slides were pre-hybridized in the hybridization buffer provided by the manufacturer (Molecular Instruments) and then hybridizedwith the indicatedprobe (20 nM, in hybridization buffer) at 37 °C overnight. ..

    Incubation:

    Article Title: Evolutionary origins and transcriptomic innovations of vertebrate Cajal-Retzius cells.
    Article Snippet: .. After rinsing in SSCT, sections were pre-hybridized in Hybridization Buffer (Molecular Instruments) for 1.5h at 37◦C and then incubated overnight with probes at 12 nM concentration in Hybridization Buffer at 37◦C. .. The following day, samples were washed in Wash Buffer (Molecular Instruments) and then in SSCT, then incubated in Amplification Buffer (Molecular Instruments) for 30 min.

    Article Title: Retinoic Acid Informs the Positional Identity of Frontonasal Neural Crest Cells Through Alx Family of Transcription Factors
    Article Snippet: .. After pre- hybridizing in 500 μL hybridization buffer (HCR Buffers, Molecular Instruments, USA) for 30 min at 37°C, the embryos were incubated overnight at 37°C in hybridization solution containing probes targeting genes (4 pmol in 500 μL hybridization buffer). .. After hybridization, samples were washed four times using HCR washing buffer (HCR Buffers, Molecular Instruments, USA) at 37°C, followed by two washes in 5xSSCT at RT.

    Article Title: Engulfment by brain macrophages in a short-lived vertebrate
    Article Snippet: .. After the overnight incubation with probes sets diluted in hybridization buffer, HCR wash buffer (Molecular Instruments, “HCR Probe Wash Buffer,” for tissue sections; 250 μL per slide; preheated to 37°C) was applied to was applied to the slides, which were incubated at 37°C for two washes of 30 minutes each with a coverslip on top. ..

    Article Title: Elevated phagocytic capacity directs innate spinal cord repair.
    Article Snippet: .. For blocking, sections were incubated in prewarmed hybridization buffer (Molecular Instruments) for 1 h at 37◦C. ..

    Article Title: Methods, culture medias and devices for generating embryos in vitro from stem cells
    Article Snippet: After at least one day of storage at −20° C., the tissue sections were permeabilized in 70% ethanol at 4° C. for >1 h, and then dried and cleared with 1 ml of 8% SDS (Invitrogen, AM9822) in 1×PBS at room temperature for 30 min. After rinsing with PBS two times and nuclease-free water one time, a custom-made flow cell (fluidic volume about 30 μl), which were made from glass slide (25×75 mm) with 1 mm thickness and 1 mm diameter holes and a PET film coated on both sides with an acrylic adhesive with total thickness of 0.25 mm (Grace Bio-Labs, RD481902) was attached to the coverslips. .. Using the 1 mm diameter holes, the tissue samples were incubated in 30% hybridization buffer (Molecular Instruments) containing 3.3 nM of each probe overnight at 37° C. The holes of the flow cells were covered using a sticker (Grace Bio-Labs, GBL629200) to prevent evaporation during incubation. .. The samples were then washed with 30% wash buffer (Molecular Instruments) for 4 times over 1 h, and then rinsed with 4× saline-sodium citrate buffer (SSC) (Thermo Fisher, 15557036) 5 times.

    Article Title: Evolutionary origins and transcriptomic innovations of vertebrate Cajal-Retzius cells.
    Article Snippet: .. Probes were added to the Hybridization Buffer (Molecular Instruments) to a concentration of 12 nM and incubated for three days at 37◦C. .. Samples were washed in Wash Buffer (Molecular Instruments) and SSCT (5xSSC + 0.1% TritonX) before incubation with 60 pM snap-cooled HCR hairpins (Molecular Instruments) in Amplification Buffer (Molecular Instruments) for 3 days at 4◦C.

    Concentration Assay:

    Article Title: Evolutionary origins and transcriptomic innovations of vertebrate Cajal-Retzius cells.
    Article Snippet: .. After rinsing in SSCT, sections were pre-hybridized in Hybridization Buffer (Molecular Instruments) for 1.5h at 37◦C and then incubated overnight with probes at 12 nM concentration in Hybridization Buffer at 37◦C. .. The following day, samples were washed in Wash Buffer (Molecular Instruments) and then in SSCT, then incubated in Amplification Buffer (Molecular Instruments) for 30 min.

    Article Title: Evolutionary origins and transcriptomic innovations of vertebrate Cajal-Retzius cells.
    Article Snippet: .. Probes were added to the Hybridization Buffer (Molecular Instruments) to a concentration of 12 nM and incubated for three days at 37◦C. .. Samples were washed in Wash Buffer (Molecular Instruments) and SSCT (5xSSC + 0.1% TritonX) before incubation with 60 pM snap-cooled HCR hairpins (Molecular Instruments) in Amplification Buffer (Molecular Instruments) for 3 days at 4◦C.

    Staining:

    Article Title: Artificial expression constructs for selectively modulating gene expression in non-neuronal brain cells
    Article Snippet: For sectioning, hemispheres were embedded in 1% low-melt agarose in PBS and cut 50 μm sagittal sections on a Leica VT1000S vibratome in cold PBS buffer. .. Sections were post-fixed in 4% PFA in PBS for 2 hours and then rinsed in PBS at room temperature, then dehydrated with 70% ethanol at 4° C. Afterwards sections could be stored for up to a month in 4° C. For staining, sections were cleared with 8% SDS in PBS for 2 hours at room temperature then washed three times in 2×SSC for 1 hour each, then with Hybridization Buffer (Molecular Instruments) in a new well before applying Hybridization Buffer containing HCR Probes and hybridized overnight at 37° C. The next day samples were washed with 30% Probe Wash Buffer for 1 hour at 37° C., then rinsed with 2×SSC. ..

    Blocking Assay:

    Article Title: Elevated phagocytic capacity directs innate spinal cord repair.
    Article Snippet: .. For blocking, sections were incubated in prewarmed hybridization buffer (Molecular Instruments) for 1 h at 37◦C. ..

    Evaporation:

    Article Title: Methods, culture medias and devices for generating embryos in vitro from stem cells
    Article Snippet: After at least one day of storage at −20° C., the tissue sections were permeabilized in 70% ethanol at 4° C. for >1 h, and then dried and cleared with 1 ml of 8% SDS (Invitrogen, AM9822) in 1×PBS at room temperature for 30 min. After rinsing with PBS two times and nuclease-free water one time, a custom-made flow cell (fluidic volume about 30 μl), which were made from glass slide (25×75 mm) with 1 mm thickness and 1 mm diameter holes and a PET film coated on both sides with an acrylic adhesive with total thickness of 0.25 mm (Grace Bio-Labs, RD481902) was attached to the coverslips. .. Using the 1 mm diameter holes, the tissue samples were incubated in 30% hybridization buffer (Molecular Instruments) containing 3.3 nM of each probe overnight at 37° C. The holes of the flow cells were covered using a sticker (Grace Bio-Labs, GBL629200) to prevent evaporation during incubation. .. The samples were then washed with 30% wash buffer (Molecular Instruments) for 4 times over 1 h, and then rinsed with 4× saline-sodium citrate buffer (SSC) (Thermo Fisher, 15557036) 5 times.



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